paragallinarum, a microorganism implicated in respiratory diseases in commercial birds. 16S rDNA Sequencing Much of microbial taxonomy and metagenomic analyses nowadays are based on studies of the bacterial 16S ribosomal RNA gene (16S). Members of the phytoplasma clade can be differentiated into at least 15 phytoplasma 16Sr DNA groups (16Sr) based on RFLP analysis of the 16S rRNA gene (Lee et al., 2000). australiense strains could be differentiated into four subgroups, named 16SrXII-B (tuf-Australia I rp-A). The ARDRA method could prove to be valuable for molecular identification of A. 16S rRNA or rDNA sequence analysis has become a major tool in the determination of relationships between bacteria, and it is widely used for identification. Sequence analysis of the tuf gene and rp operon showed that Ca. paragallinarum, as well as from Escherichia coli, a bacterium associated with infectious coryza. paragallinarum strains, but differed from those obtained for Ornithobacterium rhinotracheale, a bacterium with a high genetic and phenotypic resemblance to A. T1 - Classification of a new phytoplasmas subgroup 16SrII-W associated with Crotalaria witches broom diseases in Oman based on multigene sequence analysis. The DNA patterns with DdeI and RsaI endonucleases were identical for the 19 A. The 16S rDNA was amplified by PCR using a pair of bacterial universal primers and restriction analysis of 16S rDNA sequences was done to select endonucleases with the highest number of cutting points inside the 16S rDNA. processed on both strands using automated DNA sequencing machine. information, the 16S rDNA sequences containing internal. The 16Sr I aster yellows (AR) phytoplasma group with more than 103 phytoplasmas is the largest subclade (Seemüller et al., 1998) and has. reported a classification analysis based on the 16S rRNA sequence of an organism formerly. Sequence analysis of the 16S rRNA gene represents a highly accurate and versatile method for bacterial classification and identification, even when the. A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying hens from different regions of Peru. Sequence analysis of the 16S rDNA, 16S - 23S intergenic region and the beginning of the.
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